Characterization of 12 Polymorphic SSR Markers inVeronicaSubsect.Pentasepalae(Plantaginaceae) and Cross-Amplification in 10 Other Subgenera
نویسندگان
چکیده
منابع مشابه
Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera1
PREMISE OF THE STUDY Microsatellite primers were developed in the perennial herbs of the diploid-polyploid complex Veronica subsect. Pentasepalae (Plantaginaceae) to investigate the role that hybridization has played in the evolution of the group, which includes several endangered species. METHODS AND RESULTS Twelve pairs of primers leading to polymorphic and readable markers were identified ...
متن کاملCharacterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species1
PREMISE OF THE STUDY We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing. METHODS AND RESULTS To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an av...
متن کاملCross-species amplification of eucalyptus SSR markers in Casuarinaceae
Cross-species amplification of Simple Sequence Repeats (SSRs) loci is considered as a cost-effective approach for developing microsatellite markers for new species. We examined the transferability of eucalyptus SSR loci to the economically important members of the Casuarinaceae family. Ten primer pairs targeting (GA)n, (CTT)n, (TGA)n and (GAA)n motifs were screened in Allocasuarina littoralis, ...
متن کاملDevelopment and Characterization of Polymorphic EST-SSR and Genomic SSR Markers for Tibetan Annual Wild Barley
Tibetan annual wild barley is rich in genetic variation. This study was aimed at the exploitation of new SSRs for the genetic diversity and phylogenetic analysis of wild barley by data mining. We developed 49 novel EST-SSRs and confirmed 20 genomic SSRs for 80 Tibetan annual wild barley and 16 cultivated barley accessions. A total of 213 alleles were generated from 69 loci with an average of 3....
متن کاملCharacterization of polymorphic microsatellite markers for the Neotropical leaf-frog Phyllomedusa burmeisteri and cross-species amplification.
Twelve polymorphic tetranucleotide microsatellite loci were isolated and characterized for the leaf-frog Phyllomedusa burmeisteri, an endemic species of the Brazilian Atlantic forest. These loci were screened in 25 individuals from two populations of the Minas Gerais State (Carangola and Juiz de Fora). The number of alleles per locus ranged from 3 to 16 (mean = 8). Observed and expected he...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2015
ISSN: 2168-0450
DOI: 10.3732/apps.1500059